Analysis Note

ControlHuman spleen tissue lysate

Application

Research CategorySignaling

Research Sub CategoryDevelopmental Signaling

Anti-IL-32 Antibody is a highly specific rabbit polyclonal antibody, that targets IL-32 & has been tested in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: 10 µg/mL from a representative lot detected IL-32 in human spleen tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected IL-32 in human spleen cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin-32 (IL-32), also known as Natural killer cells protein 4 (NK4) and Tumor necrosis factor alpha-inducing factor (TAIF), may play a role in innate and adaptive immune responses. IL-32 induces various cytokines such as TNFA/TNF-alpha, MIP-2 and IL-8, and activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. IL-32 has been shown to be induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFN gamma, and it has been suggested to play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis. IL-32 is different from other known cytokine families because it does not share sequence homology with them and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. IL-32 is selectively expressed in lymphocytes and expression is more prominent in immune cells than in non-immune cells. The expression of IL-32 increased after activation of T-cells by mitogens and after activation of NK cells by IL2/interleukin-2.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human IL-32.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blot in human spleen tissue lysate.

Western Blot Analysis: 5 µg/mL of this antibody detected IL-32 in 15 µg of human spleen tissue lysate.

Specificity

This antibody recognizes two isoforms at ~14 kDa and ~22 kDa at the C-terminus of IL-32.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~14 kDa and ~22 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 5 isoforms between ~15 kDa and ~27 kDa